Zebron PLUS GC Columns - Improved Inertness, Better Peak Shape, Low Bleed, and Aqueous Stability
Souhrn
Importance of the Topic
Gas chromatography remains a cornerstone of analytical chemistry, enabling separation and quantification of a wide range of organic compounds. Enhancing column performance through improved inertness, reduced bleed, better peak shape and aqueous stability is critical for reliable results in environmental, forensic, pharmaceutical, food and industrial applications.
Objectives and Study Overview
This work presents the Zebron PLUS line of GC columns, designed to address common challenges such as active analyte adsorption, thermal degradation and phase bleed. The study evaluates various PLUS phases (ZB-1plus, ZB-5plus, ZB-5MSplus, ZB-624plus and ZB-WAXplus) across standard test mixtures and real-world analytes to demonstrate improvements over existing commercial columns.
Methods and Instrumentation
The columns were tested under controlled GC conditions including:
- Injection: split ratios from 5:1 to 200:1, injection temperatures of 140–250 °C and varied liner types (Zebron PLUS Single Taper or Straight Z-Liner).
- Carrier gases: hydrogen (1.18 mL/min) or helium (1.1–2.4 mL/min) constant flow.
- Oven programs: isothermal holds and temperature ramps covering −60 to 360 °C, depending on phase limits.
- Detectors: flame ionization detector at 250–325 °C and mass spectrometry (MSD or GC-MS/MS) between 180–230 °C.
Phase inertness and bleed were quantified by tailing factors and bleed profiles, while separations of acids, amines, pesticides, SVOCs, PAHs, residual solvents, volatile amines and aqueous analytes demonstrated real-world performance.
Key Findings and Discussion
- ZB-1plus (100 % dimethylpolysiloxane) exhibited the lowest overall column activity and bleed up to 360 °C, delivering superior signal-to-noise ratios and mass spectral integrity for non-polar and active compounds.
- ZB-5plus (5 % phenyl/95 % dimethylpolysiloxane) combined broad application versatility with improved peak shapes for acidic/basic analytes, drugs of abuse and pesticides, maintaining consistent column-to-column performance.
- ZB-5MSplus (5 % phenyl-arylene) offered further deactivation for highly sensitive GC-MS and GC-MS/MS work, reducing bleed at high temperatures and improving detection of acids, alkaloids and residual solvents.
- ZB-624plus (G43 proprietary) provided enhanced deactivation for volatile organics, terpenes, residual solvents and EPA method analytes; demonstrated low bleed to 320 °C, sharper peaks for volatile amines and shortened runtimes in residual solvent analysis.
- ZB-WAXplus (100 % polyethylene glycol) achieved exceptional aqueous stability, reproducible separations of alcohols, glycols and chlorinated solvents and maintained selectivity and inertness toward acidic compounds under repeated water injections.
- Ancillary products such as Zebron gas management filters and traps with click-on connectors ensured leak-free gas delivery and simplified filter changes without interrupting instrument operation.
Benefits and Practical Applications
- Improved inertness and low bleed enhance sensitivity and reproducibility in environmental (EPA methods), pharmaceutical, forensic and food testing.
- Wide temperature range and robust bonding allow analysis of high-boiling solvents and specialized matrices such as cannabis terpenes and essential oils.
- Aqueous stability expands capabilities for beverage analysis, glycols and other water-based samples with minimal phase degradation.
- Universal compatibility with major GC systems and simplified liner and filter installations reduce downtime and maintenance costs.
Future Trends and Opportunities
The development of increasingly inert and thermally robust stationary phases will support emerging needs in ultratrace analysis, comprehensive two-dimensional GC and rapid screening of complex matrices. Integration of inline gas purification, automated column conditioning and advanced detector coupling will further boost method sensitivity and throughput. Customized phase chemistries may address novel analytes in metabolomics, petrochemical quality control and environmental monitoring of emerging contaminants.
Conclusion
Zebron PLUS columns deliver marked improvements in inertness, bleed reduction, peak shape and aqueous stability, outperforming standard commercial phases across a broad spectrum of applications. Their versatility and robustness make them valuable tools for routine and high-sensitivity GC analyses.
References
No literature references were provided in the original material.
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Zebron PLUS
GC Columns
Improved Inertness, Better Peak Shape,
Low Bleed, and Aqueous Stability
ZB-1plus™
ZB-5plus™
ZB-5MSplus™
ZB-624plus™
ZB-WAXplus™
www.phenomenex.com/GCplus
GC Columns
™
PLUS
2
Exceptionally Versatile GC phases
Exceptional Inertness
Reduced Bleed
Improved Peak Shape
Aqueous Stability
Zebron™ PLUS Selection Table
PLUS
© 2019 Phenomenex, Inc. All rights reserved.
POLARITY
PHASE
COMPOSITION
TEMP. LIMITS
(Isothermal/TPGC)
GC-MS
CERTIFIED
USP
PHASE
APPLICATIONS
RECOMMENDED USE
FOR ALTERNATE RESULTS
Page
5 ZB-1plus
™
Low bleed phase for non-polar compounds
100 % Dimethylpolysiloxane
-60 to 360/370 °C
✔
G1, G2,
G9, G38
Acids, Amines, Diesel Fuel, Drugs, Flavors
& Fragrances, PCBs (EPA Method 1668),
Pesticides, Essential Oils
• Especially suited to high sensitivity GC-MS
• Improved signal-to-noise ratio for better
sensitivity and mass spectral integrity
• Extremely inert for active compounds
Simulated distillation:
ZB-1XT SimDist Metal
High temperatures:
ZB-1HT Inferno™
4
8 ZB-5
plus™
Versatile, low bleed, inert 5 % phenyl
phase for multi-use applications
5 % Phenyl
95 %
Dimethylpolysiloxane
-60 to 360/370 °C
✔
G27, G36,
G41
Drugs of Abuse, EPA Methods, FAMEs,
Nitrosamines, Pesticides, Phenols,
Benzodiazepines, Barbiturates, THC
Metabolites
• Highly inert for improved peak shape of
acidic/basic compounds, drugs of abuse, and
pesticides
• Maximum sensitivity for GC-MS 5 % phenyl
selectivity with improved column-to-column
performance
SVOCs, PAHs, or PBDEs:
ZB-SemiVolatiles
Drugs of abuse:
ZB-Drug-1
5
8
ZB-5MSplus™
The next generation of inertness for
specialty chemical, forensic, toxicology,
and food testing applications
5 % Phenyl-
Arylene
95 %
Dimethylpolysiloxane
-60 to 325/350 °C
✔
G27
Acids, Alkaloids, Amines, Drugs, Essential
Oils/Flavors, Halo-hydrocarbons, Phenols,
Residual Solvents, Solvent Impurities,
Pesticides/ Herbicides, Drugs of Abuse
• Specialized deactivation for versatile 5 %
Phenyl-Arylene selectivity with improved
sensitivity
• Low bleed (MS Certified) and well-suited to
high sensitivity GC-MS and GC-MS/MS work
SVOCs, PAHS, or PBDEs:
ZB-SemiVolatiles
Alternate phenyl selectivity:
ZB-5plus
6
13
ZB-624plus™
The optimal choice for the analysis of
volatile compounds for Environmental,
Pharmaceutical, Food, Cannabis, and
specialty chemicals
Proprietary
-20 to 300/320 °C
✔
G43
Cannabis, Terpenes, Residual Solvents,
Volatile Amines, EPA Method 8260, EPA
Method 524, EPA Method 624, Food,
Flavors and Fragrances, Solvent Purity,
Alcohols
• Enhanced peak shape with superior
deactivation
• Increased sensitivity for high boiling solvent
• Extremely low bleed for GC-MS
• High temperature stability (300/320 °C)
VOCs and OVIs:
ZB-WAXplus
ZB-624
7-8
52 ZB-WAX
plus™
100 % aqueous stability with high retention
of alcohols and chlorinated solvents
100 % Polyethylene Glycol (PEG)
20 to 250/260 °C
* Thicker films (≥ 1.0 µm) are rated to
230/240 °C
G14, G15,
G16, G20,
G39, G47
Alcohols, Aldehydes, Aromatics, Essential
Oils, Flavors & Fragrances, Free Fatty
Acids, Glycols, OVIs, Pharmaceuticals,
Solvents / Residual Solvents, Styrene,
Xylene Isomers
• Exceptional stability to repeated injections
• Extremely inert for acidic compounds
• Enhanced selectivity for low boiling solvents;
high retention of alcohols and chlorinated
solvents
• Increased efficiency at 20 °C
G43 phase for residual solvents:
ZB-624
Free fatty acids testing:
ZB-FFAP
9
Phenomenex
l WEB: www.phenomenex.com
3
Low bleed phase chemistry
Clean, highly inert fused silica surface due to
very few active sites
Temperature resistant and flexible coating
What’s in the Zebron™ PLUS?
Active sites on a GC column’s surface can result in analyte adsorption and degradation, which negatively
affect peak shape and response.
To reduce potential surface activity, Zebron PLUS phases (ZB-1plus, ZB-5plus, ZB-5MSplus, and ZB-624plus)
are designed with a rigorous fused silica deactivation process that improves inertness and peak shapes for
troublesome compounds
Zebron ZB-WAXplus is specially designed for 100% aqueous stability, enhanced resolution and better
reproducibility .
POLARITY
PHASE
COMPOSITION
TEMP. LIMITS
(Isothermal/TPGC)
GC-MS
CERTIFIED
USP
PHASE
APPLICATIONS
RECOMMENDED USE
FOR ALTERNATE RESULTS
Page
5 ZB-1plus
™
Low bleed phase for non-polar compounds
100 % Dimethylpolysiloxane
-60 to 360/370 °C
✔
G1, G2,
G9, G38
Acids, Amines, Diesel Fuel, Drugs, Flavors
& Fragrances, PCBs (EPA Method 1668),
Pesticides, Essential Oils
• Especially suited to high sensitivity GC-MS
• Improved signal-to-noise ratio for better
sensitivity and mass spectral integrity
• Extremely inert for active compounds
Simulated distillation:
ZB-1XT SimDist Metal
High temperatures:
ZB-1HT Inferno™
4
8 ZB-5
plus™
Versatile, low bleed, inert 5 % phenyl
phase for multi-use applications
5 % Phenyl
95 %
Dimethylpolysiloxane
-60 to 360/370 °C
✔
G27, G36,
G41
Drugs of Abuse, EPA Methods, FAMEs,
Nitrosamines, Pesticides, Phenols,
Benzodiazepines, Barbiturates, THC
Metabolites
• Highly inert for improved peak shape of
acidic/basic compounds, drugs of abuse, and
pesticides
• Maximum sensitivity for GC-MS 5 % phenyl
selectivity with improved column-to-column
performance
SVOCs, PAHs, or PBDEs:
ZB-SemiVolatiles
Drugs of abuse:
ZB-Drug-1
5
8
ZB-5MSplus™
The next generation of inertness for
specialty chemical, forensic, toxicology,
and food testing applications
5 % Phenyl-
Arylene
95 %
Dimethylpolysiloxane
-60 to 325/350 °C
✔
G27
Acids, Alkaloids, Amines, Drugs, Essential
Oils/Flavors, Halo-hydrocarbons, Phenols,
Residual Solvents, Solvent Impurities,
Pesticides/ Herbicides, Drugs of Abuse
• Specialized deactivation for versatile 5 %
Phenyl-Arylene selectivity with improved
sensitivity
• Low bleed (MS Certified) and well-suited to
high sensitivity GC-MS and GC-MS/MS work
SVOCs, PAHS, or PBDEs:
ZB-SemiVolatiles
Alternate phenyl selectivity:
ZB-5plus
6
13
ZB-624plus™
The optimal choice for the analysis of
volatile compounds for Environmental,
Pharmaceutical, Food, Cannabis, and
specialty chemicals
Proprietary
-20 to 300/320 °C
✔
G43
Cannabis, Terpenes, Residual Solvents,
Volatile Amines, EPA Method 8260, EPA
Method 524, EPA Method 624, Food,
Flavors and Fragrances, Solvent Purity,
Alcohols
• Enhanced peak shape with superior
deactivation
• Increased sensitivity for high boiling solvent
• Extremely low bleed for GC-MS
• High temperature stability (300/320 °C)
VOCs and OVIs:
ZB-WAXplus
ZB-624
7-8
52 ZB-WAX
plus™
100 % aqueous stability with high retention
of alcohols and chlorinated solvents
100 % Polyethylene Glycol (PEG)
20 to 250/260 °C
* Thicker films (≥ 1.0 µm) are rated to
230/240 °C
G14, G15,
G16, G20,
G39, G47
Alcohols, Aldehydes, Aromatics, Essential
Oils, Flavors & Fragrances, Free Fatty
Acids, Glycols, OVIs, Pharmaceuticals,
Solvents / Residual Solvents, Styrene,
Xylene Isomers
• Exceptional stability to repeated injections
• Extremely inert for acidic compounds
• Enhanced selectivity for low boiling solvents;
high retention of alcohols and chlorinated
solvents
• Increased efficiency at 20 °C
G43 phase for residual solvents:
ZB-624
Free fatty acids testing:
ZB-FFAP
9
Phenomenex
l WEB: www.phenomenex.com
4
Zebron™ ZB-1PLUS™
Lower Overall Column Activity
Activity is a key measure of column quality. ZB-1plus
columns are aggressively tested to ensure deactivation.
Our QC test below demonstrates low tailing on ZB-1plus
for even the most active compounds, like 2-ethylhexanoic
acid.
Column:
Zebron ZB-1PLUS
Dimensions:
30 meter x 0.25 mm x 0.25 µm
Part No.:
7HG-G031-11
Injection:
Split 100:1 @ 250 °C, 1 µL
Recommended Liner:
Zebron PLUS Single Taper Z-Liner™
Liner Part No.:
AG2-0A13-05 (for Agilent® & Thermo Scientific® systems)
Carrier Gas:
Hydrogen @ 1.18 mL/min (constant flow)
Oven Program:
140 °C (Isothermal)
Detector:
FID @ 325 °C
Sample:
1. Decane
2. 2-Ethylhexanoic Acid
3. 4-Chlorophenol
4. Naphthalene
5. Tridecane
6. 1-Undecanol
7. Dicyclohexylamine
8. Pentadecane
Conditions for all columns:
Columns:
As listed
Dimensions:
30 meter x 0.25 mm x 0.25 µm
Injection:
Null Injection @ 250 ºC
Recommended Liner:
Zebron PLUS Single Taper Z-Liner™
Liner Part No.:
AG2-0A13-01 (for Agilent® & Thermo Scientific® systems)
Carrier Gas:
Hydrogen @ 100 mL/min (constant flow)
Oven Program:
240 °C for 9 min to 280 °C for 6.3 min to 320 °C for 6.4 min to 340 °C for 5.8
min to 350 °C for 5.5 min to 360 °C
Detector:
FID @ 320 ºC
1
1
2
3
5
4
6
7
8
5
10 min
Test conditions were stopped at 350 °C for all competitor columns so as not to cause damage to the
stationary phase by exceeding their maximum temperature limit.
Lower Column Bleed
We tested the ZB-1plus column bleed profile against other
“1MS” columns on the market – ZB-1plus shows the lowest
bleed, even at temperatures up to 360 °C.
0
15
3
10
17
Zebron ZB-1PLUS
Agilent VF-1ms
Agilent HP-1ms
30
min
Agilent
TPGC Limits
350 ºC
ZB-1PLUS
TPGC Limit
360 ºC
ZB-1plus has lower bleed and
higher temperature limits!
Did You Know?
Using columns with a slightly smaller ID can provide better resolution,
allowing your runtime to in turn be shortened by as much as 50 %. Learn more at:
www.phenomenex.com/GCPhaseRatio
• Very low bleed (MS Certified) phase especially suited to
high sensitivity GC-MS
• Extremely inert for active compounds such as drugs,
pesticides, or acids and bases
• Improved signal-to-noise ratio for better sensitivity and
mass spectral integrity
• Equivalent selectivity to 100 % dimethylpolysiloxane
phases
Comparative separations may not be representative of all applications.
Equivalent or Better Results
Upgrade to ZB-1plus from any 100 % dimethylpolysiloxane
phase:
Agilent®
Restek®
SGE®
Supelco®
• DB®-1
• DB-1ms
• DB-1ms Ultra Inert
• HP-1
• HP-1ms
• HP-1ms Ultra Inert
• VF-1ms
• CP-Sil 5 CB
• Ultra 1
• Rtx®-1
• Rtx-1ms
• Rxi®-1ms
• BP1
• SolGel-1ms™
• SPB®-1
• SE-30
• MET-1
• MDN-1
• Equity®-1
Recommended Applications
• Acids
• Amines
• Drugs
• EPA Methods (1668)
• Diesel Fuel
• Essential Oils
• Flavors & Fragrances
• Oxygenates and GROs
• PCBs
• Pesticides
• Solvent Impurities
• Sulfur Compounds
(Light)
App ID 24538
Phenomenex
l WEB: www.phenomenex.com
5
GC Columns
Zebron™ ZB-5PLUS™
Equivalent or Better Results
Upgrade to ZB-5plus from any 5 % phenyl / 95 %
dimethylpolysiloxane phase:
Agilent®
Restek®
SGE®
Supelco®
OV®
• DB®-5
• HP-5
• HP-5ms
• HP-5msi
• Rtx®-5
• Rtx-5MS
• Rtx-5Amine
• Rxi®-5ms
• BP5
• BPX5
• MDN-5S
• SPB®-5
• Equity®-5
• OV-5
Recommended Applications
• Barbiturates
• Benzodiazepines
• Drugs of Abuse
• EPA Methods
• FAMEs
• Nitrosamines
• Pesticides
• Phenols
• THC Metabolites
6
8
10
12
14
16
18
20
22
min
1 2
3
4,5
6
7
8
12
9,10,11
13,14,15
16
1718
19
20
21
22
23
24
25
26
27
App ID 24528
Column: Zebron ZB-5plus
Dimensions: 30 meter x 0.25 mm x 0.25 µm
Part No.: 7HG-G032-11
Injection: Split 5:1 @ 240 °C, 1 µL
Carrier Gas: Helium @ 1.2 mL/ min (constant flow)
Oven Program: 60 °C to 140 °C @ 5 °C/min to 280 °C @ 10 °C/min
Detector: MSD @ 230 °C, 45-450 amu
Sample: 1. Phenol
2. 2-Chlorophenol
3. 2-Methylphenol
4. 4-Methylphenol
5. 3-Methylphenol
6. 2,6-Dimethylphenol
7. 2-Nitrophenol
8. 2-Ethylphenol
9. 2,4-Dimethylphenol
10. 3,5-Dimethylphenol
11. 2,5-Dimethylphenol
12. 4-Ethylphenol
13. 3-Ethylphenol
14. 2,4-Dichlorophenol
15. Benzoic Acid
16. 2,3-Dimethylphenol
17. 3,4-Dimethylphenol
18. 2,6-Dichlorophenol
19. 4-Chloro-3-methylphenol
20. 2,4,6-Trichlorophenol
21. 2,4,5-Trichlorophenol
22. 2,4-Dinitrophenol
23. 4-Nitrophenol
24. 2,3,4,6-Tetrachlorophenol
25. 4,6-Dinitro-2-methylphenol
26. Pentachlorophenol
27. Dinoseb
Column:
Zebron ZB-5PLUS
Dimensions:
30 meter x 0.25 mm x 0.25 µm
Part No.:
7HG-G032-11
Injection:
Split 40:1 @ 250 °C, 1 µL
Carrier Gas:
Helium @ 1.2 mL/min (constant flow)
Oven Program:
100 °C to 180 °C @ 5 °C/min to 320 °C @ 15 °C/min
Detector:
MSD @ 180 °C, 45-450 amu
Sample:
Analytes are 50 ppm in acetone
1. Dimethyl phthalate
2. Diethyl phthalate
3. Atraton
4. Simazine
5. Prometon
6. Atrazine
7. Propazine
8. Dipropyl phthalate
9. Terbuthylazine
10. Secbumetone
11. Simetryn
12. Ametryn
13. Prometryn
14. Terbutryn
15. Dibutyl phthalate
16. 4,4’-DDE
17. 4,4’-DDD
18. Di-n-hexyl phthalate
19. 4,4’-DDT
20. Dicyclohexyl phthalate
21. bis(2-Ethylhexyl)phthalate
22. Di-n-octyl phthalate
23. Ethinyl estradiol
Phenols
11
12
13
14
15
16
17
18
19
20
21
22
23
24 min
1
16558
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
Endocrine Disruptors by GC-MS
Drug Screening by GC-MS
Column: Zebron ZB-5plus
Dimensions: 30 meter x 0.25 mm x 0.25 µm
Part No.: 7HG-G032-11
Injection: Split 15:1 @ 240 °C, 1 µL
Carrier Gas: Helium @ 1.1 mL/min (constant flow)
Oven Program: 140 °C to 240 °C @ 10 °C/min for 5 min to 320 °C @
25 °C/min for 2.25 min
Detector: MSD @ 230 °C, 45-450 amu
Sample: Analytes (underivatized) are 25 ppm in dichloromethane
1. Nicotine
2. Methylecgonine
3. Ibuprofen
4. Allobarbital
5. Aprobarbital
6. Butabarbital
7. Acetaminophen
8. Phenacetin
9. Amobarbital
10. Pentobarbital
11. Secobarbital
12. Meprobamate
13. Methyl benzilate
14. Caffeine
15. Benzphetamine
16. Hexobarbital
17. Dimenhydrinate
18. Doxylamine
19. Phenobarbital
20. 8-Chlorotheophylline
21. Methapyrilene
22. Chlorpheniramine
23. Brompheniramine
24. Cocaine
25. Chlorcyclizine
26. Codeine
27. Morphine
28. Diazepam
29. Hydrocodone
30. 6-Monoacetylmorphine
31. Oxymorphone
32. Diacetylmorphine (Heroin)
4
5
6
7
8
9
10
11
12
13
14
15
16
17
min
16421
1
2
3,4
5 6
7
8
9
10
1112
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30 31
32
App ID 24541
Excellent separation
of 32 drugs!
• Highly inert—improved peak shape for acidic/basic
compounds, drugs of abuse, and pesticides
• Very low bleed (MS certified) levels provide maximum
sensitivity
• Intense QC specifications ensure column-to-column
performance
• Engineered Self Cross-linking™ (ESC) bonding results in
phase stability and high temperature limits
• Same selectivity as the 5 % phenyl / 95 %
dimethylpolysiloxane phase
App ID 24539
Phenomenex
l WEB: www.phenomenex.com
6
min
2
3
4
5
6
min
2.55
2.6
2.65
2.7
2.75
2.8
2.85
2.9
2.95
23250
23252
23253
23251
min
2.55
2.6
2.65
2.7
2.75
2.8
2.85
2.9
2.95
min
2.55
2.6
2.65
2.7
2.75
2.8
2.85
2.9
2.95
2.55
2.6
2.65
2.7
2.75
2.8
2.85
2.9
2.95
min
min
2
3
4
5
6
min
2.55
2.6
2.65
2.7
2.75
2.8
2.85
2.9
2.95
23250
23252
23253
23251
min
2.55
2.6
2.65
2.7
2.75
2.8
2.85
2.9
2.95
min
2.55
2.6
2.65
2.7
2.75
2.8
2.85
2.9
2.95
2.55
2.6
2.65
2.7
2.75
2.8
2.85
2.9
2.95
min
min
2
3
4
5
6
min
2.55
2.6
2.65
2.7
2.75
2.8
2.85
2.9
2.95
23250
23252
23253
23251
min
2.55
2.6
2.65
2.7
2.75
2.8
2.85
2.9
2.95
min
2.55
2.6
2.65
2.7
2.75
2.8
2.85
2.9
2.95
2.55
2.6
2.65
2.7
2.75
2.8
2.85
2.9
2.95
min
App ID 23250
App ID 23251
App ID 23252
App ID 23253
Better Peak Shape
Zebron ZB-5MSplus
Agilent® DB®-5ms Ultra Inert
Agilent HP-5ms Ultra Inert
Restek® Rxi®-5Sil MS
OH
OH
1,2-Butanediol
Peak Skew = 0.786
Peak Skew = 0.580
Peak Skew = 0.535
Peak Skew = 0.645
For part numbers, applications, and more information visit
www.phenomenex.com/PlusGC
Zebron™ ZB-5MSPLUS™
Column: As listed
Dimensions: 30 meter x 0.25 mm x 0.25 µm
Injection: Split 200:1 @ 250 °C, 1 µL
Carrier Gas: Helium @ 2.4 mL/min (constant flow)
Oven Program: 140 °C (Isothermal)
Detector: FID @ 325 °C
Conditions for all columns:
Equivalent or Better Results
Upgrade to ZB-5MSplus from any 5 % phenyl or 5 % phenyl-
arylene / 95 % dimethylpolysiloxane phase:
Agilent®
Restek®
Supelco®
• DB®-5ms
• DB-5ms Ultra Inert
• HP-5ms
• HP-5ms Ultra Inert
• VF-5ms
• Rxi®-5Sil MS
• SLB®-5ms
Recommended Applications
• Acids
• Alkaloids
• Amines
• Drugs
• Essential Oils
• Flavors
• Halo-hydrocarbons
• Pesticides
• Phenols
• Residual Solvents
• Solvent Impurities
Zebron ZB-5MSplus outperforms its competitors in a
column activity test that is performed by measuring the
peak shape of 1,2-Butanediol.
• The next generation of inertness for specialty chemical,
forensic, toxicology, and food testing applications
• Specialized deactivation for versatile 5 % phenyl-
arylene selectivity with improved sensitivity
• Low bleed (MS Certified) and well-suited to high
sensitivity GC-MS and GC-MS/MS work
Comparative separations may not be representative of all applications.
App ID 23250
Phenomenex
l WEB: www.phenomenex.com
7
GC Columns
Zebron 624PLUS™
Equivalent or Better Results
Upgrade to Zebron from these similar* phases:
Agilent®
Restek®
• CP-Select 624 CB
• DB-624UI Ultra Inert
• Rxi-624Sil MS
*not exact equivalent, selectivity may differ
Recommended Applications
• Cannabis
• Terpenes
• Residual Solvents
• Volatile Amines
• EPA Method 8260
• EPA Method 524
• EPA Method 624
• Food
• Flavors and
Fragrances
• Solvent Purity
• Alcohols
• Enhanced peak shape with superior deactivation
• Increased sensitivity for high boiling solvents
• Extremely low bleed for GC-MS
• High temperature stability (300/320 ºC)
Low Bleed
Engineered Self Cross-linking™ (ESC™) provides high
thermal stability with maximum column temperatures up to
300/320 °C.
Enhanced Inertness
Proprietary superior deactivation gives great peak shape
for troublesome compounds.
High Selectivity
A G43 phase that’s highly selective for polar, non-polar, low
and high boiling solvents.
Column-to-Column Reproducibility
Excellent column-to-column reproducibility well suited for
validated methods.
Temperature Limits
Push the temperature limits of traditional 624 and elute/
bake high boiling analytes at 300/320 °C.
MS Certified
Low bleed characteristics makes it the right choice for
GC-MS.
Separation of Residual Solvent Critical Pairs in Less than 15 min
24776
min
4
2
3
4
5 6
7
9
8
1
5
6
7
8
9
10
100
200
300
400
500
600
700
800
pA
App ID 24776
Shorter Residual Solvent Analysis by
GC-FID
Why wait for an hour long method. Upgrade to ZB-624plus
and get short runtime, low bleed, high temperature
resistance, and the 624 selectivity, all in one column.
Column: Zebron ZB-624plus
Dimensions: 30 meter x 0.32 mm x 1.80 µm
Part No.: 7HM-G040-31
Injection: Split 20:1 @ 200 °C, 1 µL
Recommended Liner: Zebron PLUS Straight Z-Liner™
Liner Part No.: AG2-0A03-05 (for Agilent® & Thermo Scientific® systems)
Carrier Gas: Helium @ 1 mL/min (constant flow)
Oven Program: 40 °C for 5 min to 260 °C @ 25 °C/min for 3 min
Detector: FID @ 250 °C
Sample: 1. Methanol
2. Ethanol
3. Acetone
4. IPA
5. Acetonitrile
6. DCM
7. Ethyl Acetate
8. THF
9. Toluene
What makes the PLUS in ZB-624plus?
Superior Deactivated Fused Silica
Dramatically reduces analyte adsorption,
maximizing your peak symmetry.
Highly Selective Stationary Phase
Provides excellent separation of polar, nonpolar,
low and high boiling solvents, while Engineered
Self Cross-linking (ESC) results in high-thermal
stability and low bleed.
Polyimide Coating
Flexibility and temperature resistance
(300/320 °C).
Phenomenex
l WEB: www.phenomenex.com
8
min
14
0.0E0
1.0E4
2.0E4
24790
1.0E5
9.0E4
8.0E4
7.0E4
6.0E4
5.0E4
4.0E4
3.0E4
13
12
11
10
9
8
7
6
5
4
3
2
Volatile Organic Compounds in EPA Methods 8260, 524, and 624
Improved Peak Shape of Volatile Amines
Volatile amines are challenging analytes for GC analysis. They can adsorb to even the smallest imperfections in fused silica.
ZB-624plus undergoes a superior deactivation process which minimizes active compound adsorption leading to gains in
peak response and shape.
Our high efficiency dimension and superior deactivation can stand real world samples. In addition, MS certification
provides extreme low bleed to your GC-MS analysis.
Comparison of Volatile Amines on Various 624 Columns
Zebron™ 624PLUS™ (cont’d)
App ID 24778 App ID 24783 App ID 24782
App ID 24790
pA
min
24778-82-83
20
40
60
80
100
120
140
160
180
200
220
240
260
2.0
2.5
1
2
3
3.0
3.5
4.0
4.5
5.0
Conditions for all separations:
Column: Zebron ZB-624plus
Restek Rxi-624Sil MS
Agilent DB-624UI Ultra Inert
Dimensions: 30 meter x 0.32 mm x 1.80 µm
Injection: Split 20:1 @ 200 °C, 1 µL
Recommended Liner: Zebron PLUS Straight Z-Liner™
Liner Part No.: AG2-0A03-05 (for Agilent® & Thermo Scientific® systems)
Carrier Gas: Helium @ 1.8 mL/min (constant flow)
Oven Program: 50 °C for 1 min, to 200 °C @ 20 °C/min for 5 min
Detector: FID @ 250 °C
Sample: 1. Isopropylamine
2. Diethylamine
3. Triethylamine
Volatile Amines on a Zebron ZB-624plus - 500 ppm
Volatile Amines on a Restek® Rxi®-624Sil MS - 500 ppm
Volatile Amines on a Agilent® DB®-624UI Ultra Inert - 500 ppm
Column: Zebron ZB-624plus
Dimensions: 30 meter x 0.25 mm x 1.40 µm
Part No.: 7HG-G040-27
Injection: Split 50:1 @ 230 °C, 1 µL
Recommended Liner: Zebron PLUS Straight Z-Liner™
Liner Part No.: AG2-0A03-05 (for Agilent® & Thermo Scientific® systems)
Carrier Gas: Helium @ 0.7 mL/min (constant flow)
Oven Program: 40 °C for 2 min, to 210 °C @ 17 °C/min for 3 min
Detection: Mass Spec transfer line @ 250 °C
Abundance
Volatile amine
adsorption resulting
in poor peak symmetry
GC-MS Analysis of 74
volatile organic compounds
in less than 15 min
Low bleed
Better deactivation =
Higher sensitivity for
active analytes
Phenomenex
l WEB: www.phenomenex.com
9
GC Columns
Zebron ZB-WAXPLUS™
Water Reproducibility of ZB-WAXPLUS
Historically, polyethylene glycol (PEG) phases have been unsta-
ble with aqueous samples such as beverages or glycols, resulting
in poor reproducibility and decreased lifetime. The ZB-WAXPLUS
bonding procedure results in exceptional stability to repeated in-
jections of aqueous matrices.
Enhanced Aqueous Stability
• 100 % aqueous stable, excellent for aqueous samples
• Extremely inert for acidic compounds
• Enhanced selectivity for low boiling solvents
• High retention of alcohols and chlorinated solvents
• Increased efficiency at 20 °C
Equivalent or Better Results
Upgrade to Zebron from any 100 % polyethylene glycol phase:
Agilent®
Restek®
SGE®
Supelco®
• DB®-WAX
• CAM
• HP-20M
• Carbowax 20M
• CP-Wax 52 CB
• Stabilwax®
• BP20
• SUPELCOWAX® 10
Recommended Applications
• Alcohols
• Aldehydes
• Aromatics
• Essential Oils
• Flavors & Fragrances
• Free Fatty Acids
• Glycols
• OVIs
• Pharmaceuticals
• Solvents / Residual Solvents
• Styrene
• Xylene Isomers
Reproducibility and Stability Test
Column: Zebron ZB-WAXplus
Dimensions: 30 meter x 0.25 mm x 0.25 µm
Part No.: 7HG-G013-11
Injection: Split 30:1 @ 140 °C, 0.2 µL
Carrier Gas: Helium @ 1.4 mL/min (constant flow)
Oven Program: 35 °C for 5 min to 85 °C @ 10 °C/min to 200 °C @ 25 °C/min for 1 min
Detection: FID @ 200 °C
Sample: 1. Acetaldehyde
2. Ethyl Acetate
3. Methanol
4. Ethanol
5. Propanol
6. Isobutanol
7. 2-Methylbutanol
8. 3-Methylbutanol
m in
0
2
1
2
3
4
15817
5
6
7,8
4
6
8
10
12
14
pA
0
10
20
30
40
50
60
70
80
90
No Way!
A specialized bonding procedure makes ZB-WAXplus columns ex-
ceptionally stable to replicate injections of water-based matrices,
while maintaining the selectivity that makes them the scientist’s
choice for highly polar compounds.
App ID 15817
Scottish Single Malt Whiskey
60 % Aqueous
Phenomenex
l WEB: www.phenomenex.com
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Our Technical Nerds are available nearly 24/7.
Live Chat today!
www.phenomenex.com/chat
or email
To learn what NerdLife is all about.
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11
Remarkably Inert,
Ridiculously Easy to Install,
GC Inlet Liners
Find Your GC Liner!
Search by:
• Application
• System Manufacturer
• Injection Type
• Part Number
Can your
liners do this?
www.phenomenex.com/FindLiner
Pre-installed O-ring
means you’re ready for
installation
Touchless installation
prevents contamination
Open
Install
GC Products
Phenomenex
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12
Zebron™ Gas Management Filters
No Diffusion and No Leaks with
Dual Wall Housing!
More protection and less contamination with
an inner glass wall and better safety with a
plastic outer wall.
Higher Capacity
The higher capacity of Zebron gas
management filters means less need for
replacement and an increase in system
uptime, saving you time and money.
Universal Compatibility
No need to change your existing
connecting unit. Simply remove the old
filter, replace the O-ring, click a new filter
in, and tighten the connection with the ring
Got LC-MS?
Zebron LC-MS gas filters are now available!
(See page 14 for ordering information.)
*Hydrocarbon filters do not include a color indicator
Easy Filter Installation in
Under 20 Seconds!
Avoid system downtime by attaching a new
filter to your gas line in seconds!
Never Disrupt Your Instrument!
The integrated one way valve on the Zebron
gas management base units allow for quick
and easy filter changes without disrupting
the instrument.
Easily Know When to
Replace Your Filter!
1
Color Change
The indicator on each
Zebron gas management filter
will display when it is time to
change your filter.*
2
Electronic
Indicator
The optional electronic indicator will
alert you with an audible warning on
when to change your filter based on
typical usage.
Phenomenex
l WEB: www.phenomenex.com
13
*Hydrocarbon filters do not include a color indicator
Zebron™ Gas Management Traps
Click-On Connection
The Click-on trap connectors allow inline car-
tridges to be exchanged without introducing
contaminants. Spring loaded check valves seal
when a filter is removed and open only when
a new filter has been locked in place.
Click-on Trap
Never Disrupt Your Instrument!
Attach a new filter in under 10 seconds. Once
the connecting unit is installed, you will be able
to change traps as often as needed without
interrupting the instrument.
High Pressure Durability
The Click-on trap is completely made out
of stainless steel and can withstand high
pressures. The seals at each end of the trap
will only be punctured when the Click-on
connector is locked in place.
Know When to Replace
The optional electronic indicator
displays when scheduled
replacement or maintenance is due.
Secure Wall Mount
These wall mounting clamps will EASILY help
attach a Zebron gas trap to a wall or surface.
Phenomenex
l WEB: www.phenomenex.com
14
Ordering Information
O-ring
Brass Click-on
Connector
Wall-mounting
Clamp Set
Electronic Indicator
First time ordering Zebron Click-on Gas Traps?
Be sure to order the brass or stainless
steel connector with your first trap.
¹∕8 in.) Brass
or AG6-4150
(
¹∕8 in.) SS
¹∕4 in.) Brass
or AG6-4160
(
¹∕4 in.) SS
Gas Management
Or
dering Infor
mation
Phenomenex
l WEB: www.phenomenex.com
Zebron Gas Management Filters
Part No.
Description
Unit
AG6-1040
Gas Filter Universal
ea
AG6-1070
Gas Filter Universal (Helium)
ea
AG6-1010
Gas Filter Oxygen
ea
AG6-1020
Gas Filter Moisture
ea
AG6-1030
Gas Filter Hydrocarbon
ea
AG6-1050
Gas Filter Hydrocarbon/Moisture for LC-MS
2/pk
AG6-1060
Ring Nut for Gas Filter
ea
Zebron Gas Management Traps
Part No.
Description
Unit
AG6-3140
Click-on Universal Trap
ea
AG6-3110
Click-on Oxygen Trap
ea
AG6-3120
Click-on Moisture Trap
ea
AG6-3130
Click-on Hydrocarbon Trap
ea
AG6-3150
Click-on Carbon Dioxide Trap
ea
Zebron Connecting Units
Part No.
Description
Unit
AG6-2101
1-position Connecting Unit ¹∕4 in. Brass
ea
AG6-2102
2-position Connecting Unit ¹∕4 in. Brass
ea
AG6-2103
4-position Connecting Unit ¹∕4 in. Brass
ea
AG6-2201
1-position Connecting Unit ¹∕8 in. Brass
ea
AG6-2202
2-position Connecting Unit ¹∕8 in. Brass
ea
AG6-2203
4-position Connecting Unit ¹∕8 in. Brass
ea
AG6-2204
High flow 2-position Connecting Unit for LC-MS
ea
AG6-2205
Particle Filter for LC-MS
ea
AG6-2206
O-ring Replacement for Gas Filter Baseplate
20/pk
Zebron Base Electronic Indicator and Other Accessories
Part No.
Description
Unit
AG6-3160
¹∕8 in. Brass Click-on Connector Set
2/pk
AG6-3170
¹∕4 in. Brass Click-on Connector Set
2/pk
AG6-3180
Wall-mounting Clamp Set for Gas Traps
2/pk
AG6-4150
¹∕8 in. Stainless Steel Click-on Connector Set
2/pk
AG6-4160
¹∕4 in. Stainless Steel Click-on Connector Set
2/pk
AG6-3190
O-ring Replacement Set for Gas Trap
20/pk
AG6-4110
Electronic Indicator for Gas Trap
ea
AG6-4120
Electronic Indicator for Gas Filter
ea
AG6-4130
Electronic Indicator for LC-MS Filter
ea
Part No.
Description
Unit
AG6-2301 1-position Connecting Unit ¹∕4 in. Stainless Steel
ea
AG6-2302 2-position Connecting Unit ¹∕4 in. Stainless Steel
ea
AG6-2303 4-position Connecting Unit ¹∕4 in. Stainless Steel
ea
AG6-2304 1-position Connecting Unit ¹∕8 in. Stainless Steel
ea
AG6-2305 2-position Connecting Unit ¹∕8 in. Stainless Steel
ea
AG6-2306 4-position Connecting Unit ¹∕8 in. Stainless Steel
ea
15
Ordering Information
Your happiness is our mission. Take
45 days to try our products. If you
are not happy, we’ll make it right.
guarantee
www.phenomenex.com/behappy
GC Columns
Or
dering Infor
mation
Phenomenex
l WEB: www.phenomenex.com
Zebron ZB-1plus™ GC Columns
ID (mm)
df(µm)
Temp. Limits °C
Part No.
15-Meter
0.25
0.25
-60 to 360/370
7EG-G031-11
0.32
0.25
-60 to 360/370
7EM-G031-11
30-Meter
0.25
0.10
-60 to 360/370
7HG-G031-02
0.25
0.25
-60 to 360/370
7HG-G031-11
0.32
0.25
-60 to 360/370
7HM-G031-11
60-Meter
0.25
0.25
-60 to 360/370
7KG-G031-11
0.25
1.00
-60 to 360/370
7KG-G031-22
0.32
0.25
-60 to 360/370
7KM-G031-11
Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G031-11-B.
Some exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in.
cages.
Zebron ZB-5plus™ GC Columns
ID (mm)
df(µm)
Temp. Limits °C
Part No.
15-Meter
0.25
0.25
-60 to 360/370
7EG-G032-11
30-Meter
0.25
0.25
-60 to 360/370
7HG-G032-11
0.25
0.50
-60 to 360/370
7HG-G032-17
0.25
1.00
-60 to 360/370
7HG-G032-22
0.32
0.25
-60 to 360/370
7HM-G032-11
0.32
0.50
-60 to 360/370
7HM-G032-17
60-Meter
0.25
0.25
-60 to 360/370
7KG-G032-11
Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G032-11-B. Some
exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages.
Zebron ZB-5MSplus™ GC Columns
ID(mm)
df(µm)
Temp. Limits °C
Part No.
15-Meter
0.25
0.25
-60 to 325/350
7EG-G030-11
20-Meter
0.18
0.18
-60 to 325/350
7FD-G030-08
0.18
0.36
-60 to 325/350
7FD-G030-53
30-Meter
0.25
0.25
-60 to 325/350
7HG-G030-11
0.25
0.50
-60 to 325/350
7HG-G030-17
0.25
1.00
-60 to 325/350
7HG-G030-22
0.32
0.25
-60 to 325/350
7HM-G030-11
0.32
1.00
-60 to 325/350
7HM-G030-22
30-Meter with 5-Meter Guardian™ Integrated Guard
0.25
0.25
-60 to 325/350
7HG-G030-11-GGA
30-Meter with 10-Meter Guardian Integrated Guard
0.25
0.25
-60 to 325/350
7HG-G030-11-GGC
0.25
0.50
-60 to 325/350
7HG-G030-17-GGC
60-Meter
0.25
0.25
-60 to 325/350
7KG-G030-11
Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G030-11-B. Some
exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages.
Zebron ZB-WAXplus™ GC Columns
ID (mm)
df(µm)
Temp. Limits °C
Part No.
10-Meter
0.10
0.10
20 to 250/260
7CB-G013-02
15-Meter
0.25
0.25
20 to 250/260
7EG-G013-11
0.53
1.00
20 to 230/240
7EK-G013-22
20-Meter
0.18
0.18
20 to 250/260
7FD-G013-08
30-Meter
0.25
0.25
20 to 250/260
7HG-G013-11
0.25
0.50
20 to 250/260
7HG-G013-17
0.32
0.25
20 to 250/260
7HM-G013-11
0.32
0.50
20 to 250/260
7HM-G013-17
0.32
1.00
20 to 230/240
7HM-G013-22
0.53
1.00
20 to 230/240
7HK-G013-22
60-Meter
0.25
0.15
20 to 250/260
7KG-G013-05
0.25
0.25
20 to 250/260
7KG-G013-11
0.25
0.50
20 to 250/260
7KG-G013-17
0.32
0.25
20 to 250/260
7KM-G013-11
0.32
0.50
20 to 250/260
7KM-G013-17
0.53
1.00
20 to 230/240
7KK-G013-22
Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G013-11-B. Some
exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages.
Zebron ZB-624plus™ GC Columns
ID(mm)
df(µm)
Temp. Limits °C
Part No.
20-Meter
0.18
1.00
-20 to 300/320
7FD-G040-22
30-Meter
0.25
1.40
-20 to 300/320
7HG-G040-27
0.32
1.80
-20 to 300/320
7HM-G040-31
0.53
3.00
-20 to 300/320
7HK-G040-36
60-Meter
0.25
1.40
-20 to 300/320
7KG-G040-27
0.32
1.80
-20 to 300/320
7KM-G040-31
0.53
3.00
-20 to 300/320
7KK-G040-36
Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G040-27-B. Some
exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages.
0.18 mm, 0.25 mm, and 0.32 mm are MS certified.
GC Columns
™
Trademarks
Zebron, 1plus, 5plus, 5MSplus, WAXplus, 624plus, Z-Liner, Guardian, ESC, and Engineered Self
Cross-linking are trademarks of Phenomenex. Agilent and DB are registered trademarks of Agilent
Technologies, Inc. Restek, Rtx, Rxi, and Stabilwax are registered trademarks of Restek Corporation.
Supelco, Equity, SLB, SPB, and SUPELCOWAX are registered trademarks of Sigma-Aldrich Co. LLC.
SolGel-1ms is a trademark and SGE is a registered trademark of SGE Analytical Science Pty Ltd. OV is
a registered trademark of Ohio Valley Specialty Company. Thermo Scientific is a registered trademark
of Thermo Fisher Scientific Inc.
Disclaimer
Comparative separations may not be representative of all applications. Phenomenex is not affiliated
with Agilent Technologies, Ohio Valley Specialty Company, Restek Corp., Sigma- Aldrich Co., or
Thermo Scientific.
FOR RESEARCH USE ONLY. Not for use in clinical diagnostic procedures.
© 2019 Phenomenex, Inc. All rights reserved.
www.phenomenex.com
Phenomenex products are available worldwide. For the distributor in your country/region,
contact Phenomenex USA, International Department at [email protected]
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t: +61 (0)2-9428-6444
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All other countries/regions
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t:
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Zebron™ PLUS
GC Columns
Improved Inertness, Better Peak Shape,
Low Bleed, and Aqueous Stability
BR58120819_W
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