<a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products?category=136&amp;manufacturerItems=50">Extrakce na pevné fázi</a> (SPE) je běžně volenou metodou přípravy vzorků před analýzou pomocí HPLC a GC. Poskytuje:<ul><li>Čištění vzorku;</li><li>Předkoncentraci analyzované látky;</li><li>Výměnu rozpouštědla.</li></ul>Tato metoda dobře funguje pro homogenní kapalné vzorky, avšak je zřejmé, že pevný vzorek nelze přímo aplikovat na SPE zařízení. V tomto textu se zaměříme na to, jak nejlépe přistupovat k přípravě pevných vzorků před SPE.První fáze předúpravy vzorku zahrnuje homogenizaci pevného vzorku s vhodným rozpouštědlem. Vybrané rozpouštědlo by mělo:<ul><li>Účinně rozpouštět analyt;</li><li>Účinně rozpouštět matrici vzorku (i když to není nezbytné, pokud je vzorek dobře promíchán s rozpouštědlem);</li><li>Ideálně být slabým rozpouštědlem z pohledu následující SPE fáze. To však nemusí být vždy možné, a proto může být nutné vzorek vysušit a následně rekonstituovat před aplikací na SPE kolonu.</li></ul>Po vytvoření suspenze vzorku ve vhodném rozpouštědle by měl být vzorek promíchán na vortexu pro důkladné promísení a následně centrifugován, aby se usadily nerozpustné složky matrice. Supernatant (kapalná fáze) se poté oddělí a dále zpracovává.V tomto okamžiku je nutné zvolit odpovídající SPE mechanismus:<h3>Metoda reverzní fáze SPE</h3><h4>Mechanismus / slabé rozpouštědlo / silné rozpouštědlo</h4><ul><li>Reverzní fáze – voda/pufr – acetonitril/methanol (nejsilnější je hexan)</li><li>Normální fáze – hexan/heptan – středně polární organická rozpouštědla, jako je DCM, MTBE, IPA (nejsilnější je voda)</li><li>Iontová výměna – vodný pufr s pH, které podporuje iontové výměnné interakce mezi analyzovanou látkou a sorbentem SPE – organické rozpouštědlo, obvykle methanol s úpravou pH pro narušení iontových výměnných interakcí.</li></ul>V tomto bodě je třeba vzít v úvahu jak vlastnosti analytu, tak i rozpouštědlo použité pro přípravu vzorku. Extrakce na reverzní fázi je účinná pro středně polární až středně hydrofobní analyty, ale pouze pokud jsou rozpuštěny ve slabém rozpouštědle, např. ve vodě. Pokud je vzorek rozpuštěn ve středně polárním rozpouštědle, je třeba jej buď zředit, nebo vysušit a rekonstituovat. Totéž platí pro normální fázi, kdy musí být vzorky rozpuštěny v hexanu nebo heptanu, aby bylo dosaženo účinné retence analytu během nanášení vzorku na SPE zařízení.Následující příklady mají tento princip ilustrovat:<h4>Extrakce PCB z půdy</h4>PCB jsou hydrofobní a dobře se rozpouštějí v nepolárních rozpouštědlech. Zeminu lze vysušit, zvážit a extrahovat pomocí hexanu nebo heptanu. Po důkladném promíchání a centrifugaci lze získaný roztok aplikovat přímo na kondicionovanou SPE kolonku se silikagelem, čímž se provede extrakce na &nbsp;normální fázi.<h4>Tylosin z masa</h4>Tylosin je antibiotikum s hodnotou log P = 1,0. Extrakcí masa směsí acetonitrilu a vody (80:20) dojde k narušení hydrofobních interakcí a uvolnění tylosinu. Po centrifugaci bude nutné další naředění vodou, aby se snížila síla rozpouštědla před extrakcí na reverní fázi.<h4>Enilkonazol z pomerančů</h4>Enilkonazol je fungicid běžně používaný v zemědělství, zejména u citrusových plodů. Lze jej snadno extrahovat z pomerančů pomocí acetonitrilu. Jelikož je enilkonazol zásaditý, přidání HCl do acetonitrilového extraktu podpoří protonaci analytu, což umožní jeho přímou retenci na SPE sorbentu se silnými katexovými vlastnostmi (SCX). Tento postup výrazně zjednodušuje extrakci, protože není potřeba žádné ředění vzorku (na rozdíl od extrakce na reverzní nebonormální fázi, kde by 100% acetonitril způsobil, že by se analyt během nanášení nezachytil).

Na co myslet při práci s pevnými vzorky a SPE

Moderní metody termické analýzy už dávno nejsou spojeny pouze s polymerními systémy a často přesahují rámec standardních materiálových věd. Příkladem může být použití těchto metod ve farmacii, lékařských vědách, systematické analýze životního prostředí nebo třeba právě v potravinářství. Mimo komplexní a rychlé analýzy nabízí metody termické analýzy také možnost simulace a optimalizace zpracovatelských postupů.<h4>Jak se k tomu staví Hitachi</h4>Hitachi, jakožto technologický leader, tuto skutečnost chápe a nabízí řadu aplikačních a technologických řešení v oblasti potravin. Velkou výhodou je, že získaná informace je z hlediska složení a interakcí jednotlivých analytů komplexní a díky připojitelným spektrálním technikám i chemicky specifická. Nasazení právě spektrálních technik a pokročilé analýzy obrazu činí z metod DSC a STA výkonné nástroje.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Varime_s_termickou_analyzou_b7f4843fa5.jpg" alt="Pragolab: Vaříme s termickou analýzou" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Varime_s_termickou_analyzou_b7f4843fa5.jpg 196w," sizes="100vw" width="1131" height="900"><h4>Uzenářské a masné výrobky</h4>Metody DSC (diferenční srovnávací kalorimetrie) lze s výhodou použít ke studiu složení masných výrobků z hlediska vody, tuků a proteinů a jejich chování v teplotním profilu dalšího zpracování. Tento výrobek byl tedy rozmrazen a je dostatečně tepelně zpracován. K potvrzení analýzy bylo provedeno následující měření.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Varime_s_termickou_analyzou_Obr_2_Uzenarske_a_masne_vyrobky_04052aa2af.jpg" alt="Pragolab: Vaříme s termickou analýzou - Obr. 2 Uzenářské a masné výrobky." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Varime_s_termickou_analyzou_Obr_2_Uzenarske_a_masne_vyrobky_04052aa2af.jpg 245w," sizes="100vw" width="852" height="373"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Varime_s_termickou_analyzou_Obr_3_Uzenarske_a_masne_vyrobky_0fe6e76ee2.jpg" alt="Pragolab: Vaříme s termickou analýzou - Obr. 3 Uzenářské a masné výrobky" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Varime_s_termickou_analyzou_Obr_3_Uzenarske_a_masne_vyrobky_0fe6e76ee2.jpg 176w," sizes="100vw" width="782" height="692"><h4>Původ a termální historie potravin</h4>Lokální podmínky výroby a zpracování potravin determinují jejich chování při DSC měření. Jednoduchou a levnou metodou lze tak sledovat např. změny na straně dodavatele nebo reflektovat dietní požadavky zákazníků.Zde uvádíme příklad rýže, kde hlavní měřenou složkou je obsah a stupeň želatinace škrobu.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Varime_s_termickou_analyzou_Obr_4_bf72a92f24.jpg" alt="Pragolab: Vaříme s termickou analýzou - Obr. 4 Původ a termální historie potravin (rýže)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Varime_s_termickou_analyzou_Obr_4_bf72a92f24.jpg 231w," sizes="100vw" width="261" height="176"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Varime_s_termickou_analyzou_Obr_5_373459202e.jpg" alt="Pragolab: Vaříme s termickou analýzou - Obr. 5 Původ a termální historie potravin (rýže)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Varime_s_termickou_analyzou_Obr_5_373459202e.jpg 232w," sizes="100vw" width="477" height="321">Dva endotermní píky u měření (A) znamenají obsah škrobu 20% amylóza a 80% amylopektin. U předvařené rýže, která již prošla částečnou želatinací je pík jen u 53°C. U vzorku (C) není želatinace patrná vůbec díky metodě zpracování a zmražení.&nbsp;<h4>Oxidativní stabilita chipsů a instantních jídel</h4>Použití různých typů olejů do chipsů a instantních jídel do jisté míry určuje jejich stabilitu. Údaj o stabilitě je důležitý pro určení skladování a trvanlivosti. Při zvažování kompozice výrobku nebo při hodnocení jejich stability je metoda STA (simultánní DSC a TGA termogravimetrická analýza) rychlou a snadnou metodou.Vzorky se skladují při laboratorní teplotě určitý počet dní a poté se měří v otevřené vzorkovnici za přítomnosti vzduchu při 150°C. Ukazatelem je čas, kdy nastane exotermní pík znamenající degradaci vzorku.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Varime_s_termickou_analyzou_Obr_6_fe996c754c.jpg" alt="Pragolab: Vaříme s termickou analýzou - Obr. 6 Oxidativní stabilita chipsů a instantních jídel" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Varime_s_termickou_analyzou_Obr_6_fe996c754c.jpg 207w," sizes="100vw" width="489" height="369">Z hlediska oxidativní stability je nejstabilnější palmový olej, i když je třeba vzít v potaz environmentální aspekty jeho výroby. Stejným způsobem byly měřeny i výrobky.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Varime_s_termickou_analyzou_Obr_7_948892c7b3.jpg" alt="Pragolab: Vaříme s termickou analýzou - Obr. 7 Oxidativní stabilita chipsů a instantních jídel" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Varime_s_termickou_analyzou_Obr_7_948892c7b3.jpg 149w," sizes="100vw" width="224" height="234"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Varime_s_termickou_analyzou_Obr_8_7c3bab2c53.jpg" alt="Pragolab: Vaříme s termickou analýzou - Obr. 8 Oxidativní stabilita chipsů a instantních jídel" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Varime_s_termickou_analyzou_Obr_8_7c3bab2c53.jpg 139w," sizes="100vw" width="189" height="212">Nejstabilnějším výrobkem byly chipsy, a to díky použití palmového oleje a stabilizátorů. Značný rozdíl stability u instantních nudlí mezi 0 a 7 dny je zřejmě i díky balení v inertní atmosféře.<h4>Toxicita obalů potravin</h4>Smršťovací a jiné fólie jsou dnes běžnou součástí balení potravin a jsou velmi oblíbené díky nízké hmotnosti a ceně. Některé však mohou při nevhodném použití, např. ohřevu zabalených potravin, uvolňovat nebezpečné látky. Metoda STA ve spojení s GC-MS nám snadno řekne za jakých teplot k tomu dochází.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Varime_s_termickou_analyzou_Obr_9_b09ff9b551.jpg" alt="Pragolab: Vaříme s termickou analýzou - Obr. 9 Toxicita obalů potravin" width="214" height="142"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Varime_s_termickou_analyzou_Obr_10_393734c63c.jpg" alt="Pragolab: Vaříme s termickou analýzou - Obr. 10 Toxicita obalů potravin" width="207" height="134"><h4>Závěrem</h4>Společnost Pragolab úspěšně dodává a servisně i aplikačně podporuje <a target="_blank" rel="noopener noreferrer" href="https://icpms.cz/products?category=305&amp;distributorItems=42">moderní termické analyzátory japonské značky Hitachi</a>. Tyto přístroje vykazují značnou univerzálnost a bohatost získané informace, která má své uplatnění také na poli analýzy potravin. Avšak jedná se o stále ne zcela prozkoumanou oblast s vysokým potenciálem.<blockquote>Pro více informací kontaktujte Jaroslava Kolejku na kolejka@pragolab.cz.</blockquote>

Vaříme s termickou analýzou (DSC, STA)

Shimadzu <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products/9">Nexis GC-2030</a> / <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products/835">Brevis GC-2050</a> řídicí modul umožňuje přímé ovládání tohoto GC (plynového chromatografu) pomocí <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/product/controls-agilent-icf">Agilent ICF</a> (Instrument Control Framework). Přímé ovládání znamená, že GC lze zcela ovládat z <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/product/clarity-shimadzu-edition-demo">Clarity - Shimadzu Edition Chromatography Station</a>, což umožňuje integrované ovládání GC a zajišťuje tak kompletní automatizaci laboratoří. Metoda přístroje ovládající podmínky analýzy se uloží do naměřených chromatogramů. Kromě výše uvedeného CG umožňuje ovládací modul také ovládání autosamplerů ovládaných prostřednictvím GC. Ovládání se provádí přes LAN. Minimální požadovaný firmware GC je verze 1.09.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_controls_shimadzu_nexis_gc_2030_13f4bb7b67.jpg" alt="DataApex: Řídící modul pro Shimadzu Nexis GC-2030 (na obrázku) / Brevis GC-2050" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_controls_shimadzu_nexis_gc_2030_13f4bb7b67.jpg 156w," sizes="100vw" width="700" height="700">Ovládání GC Shimadzu Nexis GC-2030 / Brevis GC-2050 je k dispozici pouze prostřednictvím speciální verze Clarity OEM s názvem <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/product/clarity-shimadzu-edition-demo">Clarity - Shimadzu Edition</a> (p/n C50-28), která je k dispozici pouze v Evropě.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/controls_shimadzu_gc_2030_2050_bc8c5fb148.pdf">Stáhněte si MANUÁL pro řídicí modul Shimadzu Nexis GC-2030 / Brevis GC-2050 zde.</a></blockquote><h4>Specifikace</h4><h5>Shimadzu Nexis GC-2030 / Brevis GC-2050</h5><ul><li>Řízené přístroje:<ul><li>Plynový chromatograf <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products/9">Nexis GC-2030</a></li><li>Plynový chromatograf <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products/835">Brevis GC-2050</a></li><li>Autosamplery řady AOC-20</li></ul></li><li>Podporované detektory: FID, TCD, FTD, FPD, ECD, BID</li><li>Komunikační rozhraní: LAN</li><li>Zakoupení:<ul><li>GC Control - (p/n A23)</li><li>GC Shimadzu Control (p/n A23-001) – je třeba zakoupit jednou pro každý připojený systém</li><li>AS Control – (p/n A26) – pokud je k dispozici autosampler</li></ul></li><li>Související produkty: Clarity – Shimadzu Edition (p/n C50-28) K dispozici pouze v Evropě</li><li>Kabel: Cross LAN (p/n SK08)</li></ul>

DataApex řídící modul pro Shimadzu Nexis GC-2030 / Brevis GC-2050

Aplikace

Akademie

Software

Video

Článek

Produkt

Česká společnost DataApex se od svého založení v roce 1991 věnuje vývoji chromatografického softwaru. DataApex se neustále snaží poskytovat uživatelům efektivní a aktuální nástroje pro zpracování chromatografických dat.

DataApex

Společnost Phenomenex je celosvětovým technologickým lídrem, který se zabývá vývojem nových řešení v oblasti analytické chemie, jež řeší požadavky analytiků na separace a purifikace v průmyslových, vládních a akademických laboratořích.

Phenomenex

Jsme jedním z lídrů na českém a slovenském trhu v oblasti laboratorních zařízení a svým zákazníkům přinášíme špičkové přístroje z oboru analytické chemie a fyzikálních měření více než 25 let.

Pragolab

Separation Science

Metrohm

Merck

Agilent Technologies

LECO

Thermo Fisher Scientific

Shimadzu Corporation

Impure gases pose a significant threat to your lab's efficiency and data integrity, leading to frustrating installation delays, costly premature instrument failure, and ultimately, flawed results. Even when you invest in high-quality gases, unexpected leaks can compromise your system.Join us for this informative webinar where we'll reveal the critical role of gas purification in optimizing your system performance. We'll explore various purification options and equip you with the knowledge to identify gas contamination in your chromatography, acting as a vital form of insurance for your lab. Don't let impure gases undermine your work—learn how to safeguard your instruments and ensure reliable data.<blockquote>Presenter: Dr. Rajni Mujoo (Technical Support Specialist - CSD, Agilent Technologies India Pvt. Ltd.)Dr. Rajni has been working with Agilent Technologies as a Technical Support Specialist in the Chemistries and Supplies Division since 2011. She holds a Ph.D. in Food Science from CFTRI, Mysore, and completed her Postdoctoral studies in the Department of Food Science at Michigan State University.Dr. Rajni has an impressive academic record, including four international presentations and 13 publications in renowned national and international journals. With a total of 16 years of experience spanning research and sales, she has developed expertise in various instrumental techniques.</blockquote>

Agilent Technologies: Prevent, Protect, Purify: The Benefits of Gas Filtration

Prevent, Protect, Purify: The Benefits of Gas Filtration

Join us for this informative webinar where we'll reveal the critical role of gas purification in optimizing your system performance

In Part 2 of this workshop, we look at performing measurements on the Cary 5000 UV-VIS/NIR Spectrophotometer with the Praying Mantis accessory from Specac/Harrick. &nbsp;The unique design of this accessory is key for assessing the diffuse reflectance of small volumes of powder. &nbsp;We will discuss installation of the Praying Mantis accessory and show setup of the Cary 5000 for measurement.<blockquote>Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.</blockquote>

Agilent Technologies: Optimize your FTIR, UV-Vis-NIR, Raman, Imaging, and Fluorescence Workflows

Part 2: Characterizing catalysts using an UV-VIS/NIR system with the Praying Mantis diffuse reflectance accessory.

In Part 2 we look at performing measurements on the Cary 5000 UV-VIS/NIR Spectrophotometer 

Catalyst Analysis with UV-VIS/NIR & Praying Mantis DR

Join us for an insightful webinar on the advantages and best practices of using Hydrogen as a carrier gas with Gas Chromatography – Mass Spectrometry (GC/MS) instruments. This session will discuss several topics of critical importance when converting from Helium to Hydrogen carrier.Key Topics include:<ul><li>Safety considerations when using Hydrogen</li><li>Selecting the appropriate ion source</li><li>Choosing the right column for Hydrogen carrier gas</li><li>Understanding the effects of Hydrogen on analytical outcomes</li></ul>Whether you are new to GC/MS or looking to enhance your current setup, this webinar will equip you with the knowledge and tools to make informed decisions and improve your analytical results.<blockquote>Presenter: Alex Graettinger (GC/MS Data Acquisition Product Support Agilent)</blockquote>

Agilent Technologies: Becoming a Better Chromatographer - Educational Webinar Series

What to Consider for Success when Transitioning to Hydrogen Carrier Gas with GC/MS Instruments

Join us for an insightful webinar on the advantages and best practices of using Hydrogen as a carrier gas with GC/MS instruments. 

Transitioning to Hydrogen Carrier Gas with GC/MS

Developing an effective gas chromatography (GC) method requires careful consideration of several interrelated parameters. Adjusting these parameters systematically can significantly enhance analytical performance, reduce troubleshooting, and streamline workflows.This webinar explores the method development process and GC parameters to optimize, emphasizing the carrier gas, inlet, column, and detector, and identifying the ideal sequence for adjusting each.<h5>By attending this presentation, you will learn:</h5><ul><li>strategies for method development</li><li>how to optimize for sensitivity or to reduce carryover</li><li>how to break the method down into tasks</li><li>about the parameters and how to optimize them for each method task.</li></ul>Plus, with an extensive Q&amp;A session, you'll have the opportunity to ask questions and gain deeper insights from our expert.<blockquote>Presenter: Diane Turner, FRSC (Director and Senior Consultant, Anthias Consulting, UK)A Warwick University graduate, Diane completed her MSc in analytical chemistry and started her career in environmental then agrochemical science, later gaining experience as an applications chemist. Diane has developed methods and training within most industries globally for over 25 years. A Visiting Fellow at The Open University, she continues disease diagnosis research from her PhD, along with food, drug, and space applications. Diane is Past-President of the Royal Society of Chemistry (RSC) Analytical Sciences Community and Past-Chair of the Analytical Chemistry Trust Fund. Diane is a Trustee of the Recycling Organisation for Research Opportunities (RORO), amongst other committee memberships. Diane is co-author of 'Gas Chromatography-Mass Spectrometry: How Do I Get the Best Results?' and on the Editorial Board of the book series ‘Practical and Technical Guides for Lab-based Chemists’.</blockquote>

SeparationScience: Expert Answers: GC Method Development

Expert Answers: GC Method Development

Join this expert-led presentation and Q&A to expand your knowledge of method development for GC and GC-MS with these useful strategies.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>Mass spectrometry (MS) users need software solutions that make it easier to work with complex data while ensuring accurate peak integration and quantitation. Often this comes with limitations around data management, access, and compliance. The new Thermo Scientific™ Chromeleon™ 7.4 software solves these challenges. It will take targeted MS quantitation to the next level, controlled and accessed from anywhere, with native control for Thermo Scientific MS instruments. It provides MSn data support for wide range of applications, network scalability and resilience, and adherence to the most stringent regulatory requirements, all with a single software solution.Join our webinar to learn how Chromeleon 7.4 software provides MS labs with advanced tools for their targeted quantitation workflows by combining the powerful network and compliance capabilities of Chromeleon software with industry-leading Thermo Scientific MS instrumentation to deliver a best-in-class solution.Key Learning Points:<ul><li>How to streamline targeted MS quantitation workflows with Chromeleon 7.4 software</li><li>Ways to manage complex MS data securely, reducing training and administration effort</li><li>How to work remotely with secure access to MS data and instruments from anywhere</li></ul>Who should attend:<ul><li>Mass spectrometry users in environmental, food safety, and (bio)pharma labs</li><li>Lab managers and analysts focused on productivity and data compliance</li><li>Current Chromeleon software users looking to maximize targeted MS quantitation capabilities</li></ul><blockquote>Presenter: Richard Law (Product Manager, Thermo Fisher Scientific)With a background in analytical chemistry and mass spectrometry, Richard is the Product Manager for targeted MS using Chromeleon CDS. He has extensive experience developing applications using LC and GC–MS technologies, especially GC-triple quadrupole MS/MS and HRAM, and has worked across several industry segments.Presenter:&nbsp;Anthony Crawshaw (Product Manager, Thermo Fisher Scientific)With a background in applied chemistry, Anthony brings over 25 years of experience in pharmaceutical analysis and laboratory software. Since 2011, he's helped customers deploying Chromeleon enterprise systems, focusing on streamlining lab operations and ensuring smooth software deployments. Anthony is now the Product Manager for Chromeleon Enterprise software.Presenter:&nbsp;Laércio Fernandes (Product Marketing Manager, Thermo Fisher Scientific)</blockquote></div></div></div>

Thermo Fisher Scientific: Targeted MS quantitation - Controlled and accessed from anywhere

Targeted MS quantitation - Controlled and accessed from anywhere

Join webinar to learn how Chromeleon 7.4 software provides MS labs with advanced tools for their targeted quantitation workflows.

Targeted MS quantitation with Chromeleon 7.4 software

<h4>GC×GC-HRTOFMS: (1) QC approaches for volatile molecule analysis and (2) A case study on the detection of TB using sputum volatiles</h4>Grant S. Ochoa, Graham E. Browse, Jane E. HillIn this presentation we discuss (1) the need for quality control of breath and other volatile sample analysis and (2) present a case study of the volatile analysis of sputum.(1) Biological volatile samples carry intrinsic variation, thus external methods of quality control which assess the instrument and sampling variation are necessary to assess data integrity. In this presentation, I will share common quality control approaches such as the use of external standards, internal standards, check samples, and continuing calibration verifications, which can be utilized to demonstrate instrument reliability throughout sample analysis.(2) Tuberculosis (TB) is the world’s deadliest infectious disease, claiming more than 1 million lives each year, largely in low- and middle-income countries. The lack of cheap, rapid diagnostics stands in the way between curbing transmission as well as between patients and treatment in many communities. Volatile analysis represents the potential for a rapid, non-invasive test, with the APOPO organization demonstrating rats can distinguish TB by sniffing the headspace of human sputum (N = 24,000 patients[1]). To identify the biomarkers associated with this approach, the headspace of 100 sputum samples obtained from the Foundation for Innovative New Diagnostics (FIND) biobank were analyzed with SPME-Arrow – GC×GC-HRTOFMS. First, we optimized headspace extraction using SPME Arrow. Using that method, from the full set of volatile metabolites detected, partial least squares – discriminant analysis (PLS-DA), was applied to select a panel of 14 features which were optimal for distinguishing culture-positive sputum from negative. The cross-validated PLS-DA model yielded an AUC of 0.92 and a sensitivity of 0.9 and a specificity of 0.84. These results suggest that volatile biomarkers which distinguish tuberculosis are present in sputum and might contribute to a breathprint for tuberculosis that we are actively exploring. Specifically, this work continues through the creation of the Shirley Diagnostics’ TB Breath Atlas which is based on the analysis the volatile molecules breath from over 1,000 patients.References[1] Kannan R, et al. Scientific Reports 2021, 11:1, <a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41598-021-81086-x">https://doi.org/10.1038/s41598-021-81086-x</a><blockquote>Presenter: Grant Ochoa (Shirley Diagnostics, Inc.)</blockquote>

LECO: Quality Control Approaches for Volatile Molecule Analysis Using GCxGC-HRTOFMS, Case Study for TB Sputum Volatiles

Quality Control Approaches for Volatile Molecule Analysis Using GCxGC-HRTOFMS, Case Study for TB Sputum Volatiles

Learn QC strategies for volatile analysis and explore case study on TB detection in sputum using GC×GC-HRTOFMS. 

Volatile Analysis in TB Sputum via GCxGC-HRTOFMS

From food and beverages to perfumes, household products, and beyond, aroma analysis is becoming increasingly crucial for enhancing product formulation, ensuring quality control, meeting regulatory requirements, and maintaining sensory consistency.In this latest session of Shimadzu’s Digital Classrooms, explore how Shimadzu’s advanced GC-MS systems, Aroma Database, along with other innovative solutions can streamline conventional aroma analysis with accuracy. Together with our experts, gain an in-depth understanding of GC-MS principles, review real-world case studies, and witness how Shimadzu’s technologies are enabling industries to drive efficient quality control and accelerate R&amp;D efforts in aroma analysis.Don’t miss this opportunity! Register now and discover the latest insights with us.<h5>Learning Objectives:</h5><ul><li>Explore advanced GC-MS methodologies for analyzing aroma profiles with precision.</li><li>Discover Shimadzu's solutions designed to enhance identification sensitivity and improve analytical accuracy.</li><li>Examine recent advancements in GC-MS technology and evaluate their transformative potential in aroma analysis workflows.</li></ul><blockquote>Presenter: Elgin Ting Guo Wei (Senior Application Specialist (GC/GCMS), Shimadzu (Asia Pacific) Pte Ltd)Elgin received his B.Sc. in Chemistry and Biological Chemistry from Nanyang Technological University, Singapore (NTU). Since joining Shimadzu Asia Pacific in 2016, he has leveraged his expertise in gas chromatography (GC) and gas chromatography-mass spectrometry (GCMS) to address customer application challenges. Over the past nine years, Elgin has also provided training and developed applications involving accessories such as headspace, pyrolyzer, and thermal desorption for users across various industries.Presenter:&nbsp;Elvi Horiyanto (Application Specialist (GC/GCMS), Shimadzu (Asia Pacific) Pte Ltd)Elvi graduated with an Honours Bachelor of Science in Chemistry from the National University of Singapore (NUS) in 2016. She has since built a successful career in the field of analytical chemistry, specializing in gas chromatography (GC) and gas chromatography-mass spectrometry (GCMS). Over the past five years, Elvi has focused on developing innovative applications while delivering operational training and exceptional customer support, consistently achieving high customer satisfaction.Presenter:&nbsp;Chua Chun Kiang (Senior Product Specialist (GCMS), Shimadzu (Asia Pacific) Pte Ltd)Chun Kiang received his Ph.D. in Chemistry from Nanyang Technological University, Singapore. He has been at Shimadzu Asia Pacific since 2019. He has accumulated over 15 years of experience in research and training across various disciplines, including mass spectrometry, gas and liquid chromatography, nanomaterials, electrochemistry, and organic chemistry. Chun Kiang is passionate about sharing his technical knowledge and assisting others in identifying solutions to challenges in the field of chemistry.</blockquote>

Shimadzu: Guide To Aroma Analysis: Discover The Latest GCMS Solutions

Guide To Aroma Analysis: Discover The Latest GCMS Solutions

Explore how GC-MS systems, Aroma Database, along with other innovative solutions can streamline conventional aroma analysis with accuracy.

Aroma Analysis: Latest GCMS Solutions

<div id="contentLeft"><div id="realLeftContent"><div id="summary2" dir="ltr" aria-live="off"><div><div dir="" aria-atomic="true">Manufacturers across industries face increasing pressure to enhance process control, reduce variability, and ensure consistent product quality. Online spectroscopy techniques such as Raman and NIR offer powerful, real-time, non-destructive analysis, but successful implementation isn’t always straightforward. Challenges like uncertainty around application feasibility, poorly designed sampling interfaces, weak or overfitted calibration models, integration hurdles, and long-term reliability concerns can limit effectiveness.This webinar will provide practical guidance for overcoming these barriers. Attendees will gain insights into identifying suitable applications, defining technical requirements for robust interface design, developing reliable prediction models, and integrating spectroscopy into existing control systems, all to support confident decision-making and enhance process performance.<h5>This webinar is ideal for:</h5><ul><li>Production and Operations Leaders – including Production Managers, Plant Managers, and Operational Managers</li><li>Technical and Quality Experts – such as Process Engineers, Quality Control Specialists, and Automation &amp; Control Engineers</li><li>R&amp;D and Innovation Professionals – including R&amp;D Scientists, Chemometricians, and Product Development Scientists</li><li>Strategic Decision-Makers – such as Project Leads and Technical Stakeholders</li></ul><h5>Key learning points</h5><ul><li>Implementation Strategy: Learn how to assess feasibility, avoid common pitfalls, and plan for long-term success with online spectroscopy.</li><li>System Design &amp; Integration: Explore best practices for sampling interface design and seamless integration with DCS/PLC systems.</li><li>Calibration &amp; Reliability: Understand how to develop robust calibration models and maintain analyzer accuracy through monitoring, diagnostics, and lifecycle support.</li></ul><blockquote>Presenter: Dr. Sylvie Roussel (CEO, Ondalys)Dr. Sylvie Roussel holds MS degrees in Agricultural Engineering and Control/Robotics, as well as a PhD in Machine Learning from SupAgro Montpellier. She began her career as a Research Associate at Iowa State University, applying machine learning to NIR spectroscopy.Since 2003, she has served as CEO of Ondalys, a consulting and training firm specializing in chemometrics, machine learning, and AI for instrumental data such as spectroscopy, imaging, and chromatography. Ondalys supports industries including pharma, biotech, chemistry, and agrifood by providing calibration development services, model training and coaching, and reselling software tools such as SOLO/PLS_Toolbox, SIMCA®, MODDE, and DesignExpert.From 2009 to 2023, Dr. Roussel also co-founded and led INDATECH, a manufacturer of spectroscopic sensors for PAT, now part of the Chauvin-Arnoux Group. She currently serves on the boards of HélioSPIR and GFSV and contributes to EU projects as an expert in machine learning and optics.Presenter:&nbsp;Elena Hagemann (Process Spectroscopy Product Manager, Metrohm USA)Elena Hagemann is the Product Manager for Process Spectroscopy at Metrohm USA in Riverview, Florida. She earned her Master degree in Analytical and Bioanalytical Chemistry at the Applied University of Aalen, Germany, where she completed her studies with her Master thesis focused on Karl Fischer Titration, NIR Spectroscopy and Multivariate Data Analysis. Elena joined the Metrohm Group in August 2013 and started with Metrohm USA in January of 2018, focusing on Raman and NIR Spectroscopy. She performs internal as well as customer training.</blockquote></div></div></div></div></div>

Metrohm: Best Practices for Implementing Online Spectroscopy

Best Practices for Implementing Online Spectroscopy

Overcome barriers to online Raman/NIR spectroscopy and ensure robust integration, calibration, and control for consistent product quality.

Join us for an enlightening webinar series designed to empower academicians and researchers by exploring the latest advancements and opportunities in pharmaceutical sciences. At Agilent, we recognize the unique challenges faced by academicians, researchers, and students. At PharmaFrontiers, we have curated a series of content tailored to address your needs and unlock new opportunities.The PharmaFrontiers seminar series is designed to provide you with valuable knowledge, insights, and practical skills.Topics to be covered:<ul><li>Enhancing Purity and Impurity Analysis with Advanced GC-MS/MS Hardware and Software Innovations.</li><li>Impurities in Pharmaceuticals.</li><li>TBA</li></ul><blockquote>Presenter: Dr. Pramod Kumar Ragham (Director, Analytical Services Department, Daicel Chiral Technologies India Pvt. Ltd.)With over 24 years of experience in the field of Analytical Chemistry, He has been serving as the Director of the Analytical Services Department at Daicel Chiral Technologies India Pvt. Ltd. since 2017. He holds a Master's degree from Pune University and a Ph.D. from JNTU Kakinada, and has built a diverse portfolio in API and formulation development, including various dosage forms for DMF/ANDA filings, as well as managing regulatory compliance and deficiencies in analytical activities.Before joining Daicel, he held key positions at prominent organizations, including Nektar Therapeutics, Dr. Reddy’s Laboratories, Biocon, Cipla, and Orchid Pharmaceuticals. Notably, he conducted over 400+ extractables and leachable studies and also has expertise in nitrosamine-related development using advanced hyphenated techniques.As an accomplished researcher, he has published seven papers in international journals and actively participates in international conferences, including CPhI events.Presenter: Dr. Narayan Kamble (Product Specialist - GC &amp; GC/MS Agilent Technologies)Dr. Narayan Kamble is a Ph.D. in food and pesticide research and has published his work in many reputable national and international journals over the last 17 years. Also, he has a patent for the extraction of anthocyanin from Medica Black grapes. Currently he is working as a product specialist at Agilent Technologies, India. He is responsible for pre-sales customer support in the field of GC and GC Mass Specs, same time, he is responsible for customer support for method development, validation, and implementing challenging methods in food and pharma testing. He was earlier associated with PerkinElmer India Pvt. Ltd. for the mass spec business. Also, he was associated with Vimta Lab as a group leader and responsible for method development, regulatory requirements, and food sample analysis.Applications of the implementation of gas and liquid chromatography mass spectrometry (GC-MS/MS &amp; LC-MS/MS) in different markets such as Pharmaceuticals, Research, Food Quality &amp; Safety Testing, Environmentalapplications,etc.Presenter: Dr. Soma Dasgupta (Application Engineer - GC/MS, Agilent Technologies)With over 17 years of extensive experience in gas chromatography, Dr. Soma has worked across diverse applications of this analytical technique, addressing challenges in sectors ranging from food safety to pharmaceutical analysis. Her academic journey includes a doctorate from the University of Pune, where she focused on developing comprehensive methods for analyzing agrochemicals in fruits and vegetables.</blockquote>

Agilent Technologies: Pharma Frontiers Live Webinar Series: Analytical Techniques, Advances in Chromatography and Regulatory Compliance

Ensuring Excellence: Cutting-Edge Techniques for Pharmaceutical Analysis [GC/GCMS]

Learn how advanced GC-MS/MS tools improve impurity profiling in pharma. Explore hardware and software innovations for accurate, regulatory-compliant results.

GC/GCMS Pharmaceutical Analysis

<h5>In this webinar, you will learn:</h5><ul><li>How plug-and-play models embedded into a Raman analyzer simplify the calibration workflow</li><li>Applications of ready-to-use models in monitoring glucose, lactate, and monoclonal antibody (mAb) titer during cell culture runs</li><li>How the Transfer Learning Algorithms (TLA) refines and improves the initial models</li><li>How to streamline the implementation of Raman technology in Chromatography and Tangential Flow Filtration (TFF)</li></ul><h5>Detailed description:</h5>Raman spectroscopy is an effective analytical technique for in-line, real-time monitoring of cell culture parameters, requiring a calibration phase that can be resource-intensive and time-consuming. Ready-to-use models directly embedded into the Raman analyzer simplify calibration. Users can bypass extensive model development and begin monitoring for immediate insights.This webinar presents findings from fed-batch and perfusion cell culture runs utilizing ready-to-use models for in-line, real-time measurement of glucose, lactate and mAb titer. The capabilities of on-line Raman sensor for critical attributes monitoring in downstream &amp; new modalities areas will be also highlighted.An on-demand version of this webinar will be available after the live event using the same link. Register now and access the webinar at your convenience.<blockquote>Presenter: Fabrice Thomas (Senior R&amp;D Manager - PAT Raman Technology)Fabrice Thomas has more than 10 years of experience in the development of cutting-edge spectroscopic technologies for challenging industries and in complex R&amp;D project management. As Sr. R&amp;D Manager for Process Analytical Technologies (PAT) at our company, Fabrice's work focuses on the development and implementation of a GMP-ready Raman PAT platform to facilitate process monitoring in the biopharma industry from process development to manufacturing. He holds a Master Degree in Engineering Physics and a Ph.D from University Grenoble Alpes, France.Presenter:&nbsp;Kevin Grollier (PAT Quality Implementation and Support Engineer)Kevin Grollier graduated from the Lyon Pharmaceutical Industry Institute (IPIL), with an M.S. in pharmaceutical production specializing in biotechnology. At our company, he is a PAT implementation and support engineer in charge of implementing Raman projects at customer sites, training ProCellics™ Raman Analyzer users, and providing technical support on hardware, software, and data analysis topics. Before joining our company, he worked at Merial (now BIAH) as a QA validation engineer where he had the opportunity to work on various validation projects.</blockquote>

Merck: Ready-to-Use Raman for Upstream Monitoring — Expanding Insights into Downstream & Novel Modalities

Ready-to-Use Raman for Upstream Monitoring — Expanding Insights into Downstream & Novel Modalities

Discover how ready-to-use Raman models simplify calibration and enable real-time monitoring of glucose, lactate, mAb titer in bioprocessings.

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